The rice mitochondrial iron transporter is essential for plant growth

نویسندگان

  • Khurram Bashir
  • Yasuhiro Ishimaru
  • Hugo Shimo
  • Seiji Nagasaka
  • Masaru Fujimoto
  • Hideki Takanashi
  • Nobuhiro Tsutsumi
  • Gynheung An
  • Hiromi Nakanishi
  • Naoko K. Nishizawa
چکیده

In plants, iron (Fe) is essential for mitochondrial electron transport, heme, and Fe-Sulphur (Fe-S) cluster synthesis; however, plant mitochondrial Fe transporters have not been identified. Here we show, identify and characterize the rice mitochondrial Fe transporter (MIT). Based on a transfer DNA library screen, we identified a rice line showing symptoms of Fe deficiency while accumulating high shoot levels of Fe. Homozygous knockout of MIT in this line resulted in a lethal phenotype. MIT localized to the mitochondria and complemented the growth of Δmrs3Δmrs4 yeast defective in mitochondrial Fe transport. The growth of MIT-knockdown (mit-2) plants was also significantly impaired despite abundant Fe accumulation. Further, the decrease in the activity of the mitochondrial and cytosolic Fe-S enzyme, aconitase, indicated that Fe-S cluster synthesis is affected in mit-2 plants. These results indicate that MIT is a mitochondrial Fe transporter essential for rice growth and development.

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عنوان ژورنال:

دوره 2  شماره 

صفحات  -

تاریخ انتشار 2011